In the clip (which also pays slight homage to Woody Allen's "Manhattan" with the inclusion of Gershwin's "Rhapsody in Blue"), Homer, Bart, Marge, Lisa and Maggie are seen sitting as guests on Letterman's talk show, although the host seems none too impressed.
"So what are we doing here? Is it the thing where the family runs in and sits down?," Letterman asks the family. "We flew you in and put you up for that? I think we're done here."
Letterman than cues Paul Shaffer and the CBS Orchestra, as they launch into a rendition of of Danny Elfman's iconic "Simpsons" theme song.
Now in its 25th season, "The Simpsons" airs Sundays at 8 p.m. ET on Fox.