The video features replays of Michelle Obama saying that "for the first time in my adult life, I am really proud of my country."
Sen.criticized the Tennessee GOP as "low class" for posting the video. Michelle Obama has said her comments were misconstrued.
Tennessee Republican Party spokesman Bill Hobbs declined comment when contacted by the Chattanooga Times Free Press.
Corker's chief of staff, Todd Womack, said in a statement that their office has "strongly encouraged the national party and state parties to absolutely refrain from getting involved in negative personal campaigning."
Sen. Lamar Alexander's spokesman, Lee Pitts, was also critical of the ad. When asked he said, there are probably better ways the party to communicate its pride in America.