The interview is scheduled to air Wednesday on Winfrey's talk show, a spokeswoman for Harpo Productions Inc. said Monday. It will be Madonna's first TV interview about the adoption.
Madonna traveled to Malawi on Oct. 4 with her husband, Guy Ritchie. They spent eight days visiting orphanages she is funding through her charity.
The child, David Banda, was taken to London last week after Malawi's High Court granted Madonna and Ritchie an interim adoption order.
Madonna, 48, has said she acted according to the law, but the toddler's father, Yohane Banda, said Sunday he didn't realize he was signing away custody of his motherless son "for good."
"Our understanding was that they (Madonna and British filmmaker husband Guy Ritchie) would educate and take care of our son, just as they were doing at the orphanage," the 32-year-old illiterate peasant farmer told The Associated Press in a telephone interview Sunday from Lipunga Village, where he ekes out a living growing onions and tomatoes.
Until now, Banda has defended his move as in the best interests of his motherless son and criticized local charities who have started legal proceedings to challenge the adoption.
Madonna has two children — daughter Lourdes, 9, and son Rocco, 6.