The duo has landed Kenny Chesney, Rascal Flatts, Reba McEntire, Brad Paisley, Carrie Underwood, Keith Urban, George Strait, Taylor Swift, Miranda Lambert and Lady Antebellum for "ACM Presents: Brooks & Dunn - The Last Rodeo."
The special will be filmed April 19 in Las Vegas - the day after the ACM awards - and aired May 23 on CBS.
Kix Brooks and Ronnie Dunn announced last year they were breaking up after one of country music's best-selling runs.
Their last performance together will come Aug. 10 in Nashville.
Others slated to perform on the special include Sugarland, Jason Aldean and Jennifer Hudson.