In this latest film, directed by Sam Raimi, Maguire's Peter Parker is juggling his photography job, schoolwork, love life and superhero-dom. Then he gains fresh powers when his blue-and-red Spidey costume turns black and his dark side asserts itself, leaving him tangled in an inner struggle just as two new villains, Sandman (Thomas Haden Church) and Venom (Topher Grace), turn up.
Parker also has the enmity of his former best friend, Harry Osborn (James Franco), to deal with. Fans will remember that Parker was to blame for the death of his friend's father, who also happened to be an evil genius.
Maguire has been coy about whether this is his final "Spider-Man" movie.
Photos: Phil "Spider Man 3" In Tokyo
"I feel like this film is kind of a natural conclusion to a lot of stuff that's happened in the first two movies," Maguire said.
"It kind of feels like a natural trilogy," the 31-year-old actor said, adding that he's open to donning the Spider-Man suit again "if a story presents itself, if we come up with something we feel deserves to be told."
"I'm not going to make them just because they've been successful," Maguire said.
Columbia Pictures' "Spider-Man 3" opens to general audiences May 1 in Japan and May 4 globally.