Fans paid $75 to see the actors and attend the screening, which raised $260,000 for Variety of Eastern Tennessee, a charity benefiting children with disabilities.
Knoxville Mayor Bill Haslam proclaimed "Jessica Alba and Dane Cook Day" on Tuesday, and Regal Entertainment Group CEO Mike Campbell unveiled commemorative tiles to be placed at the Walk of Fame at Regal's Pinnacle 18 theater.
"Good Luck Chuck" opened in theaters Friday. Alba, 26, plays Cam, a clumsy penguin-keeper who captures Cook's heart.
Knoxville-based Regal Entertainment Group has hosted several screenings to benefit Variety, including a showing of "Talladega Nights" with Will Ferrell and "The Dukes of Hazzard" with Jessica Simpson, Seann William Scott and Knoxville native Johnny Knoxville.
Alba gained fame as an action star, first on TV's "Dark Angel," then in films such as "Fantastic Four" and "Sin City."
Cook, who began as a comedian, has starred in "Employee of the Month," with singer-actress Jessica Simpson as well as hosting Saturday Night Live and doing standup for HBO specials.