Kelley Paul held a press conference in Bowling Green to defend her husband's Christian faith, saying the two have been members of a local Presbyterian church for about 18 years.
Since late last week, Democrat Jack Conway has been airing an ad that focuses on allegations that Paul was affiliated during college with a group that mocked Christianity and that he worshipped a god he called "Aqua Buddha." Kelley Paul called it "a desperate, shameful attack on our family."
Ky. Sen. Race: Rand Paul (R) vs. Jack Conway (D)
CBS News Complete Coverage: Election 2010
The odd topic has so dominated the news in Kentucky that it's been difficult for either candidate to expound on other issues.
Rand Paul told syndicated radio talk show host Laura Ingraham on Wednesday he doesn't remember the episode described by the anonymous woman, or the woman.
"I wish I had a great story about it, but I don't remember anything about this story," he told Ingraham.
"It wasn't some big thing that any of us remember. I mean I talked to other people that were, you know, part of these hijinks, and none of them remember this woman or any of her characterization of it."
One of the heavy-hitters of Kentucky's Democratic party, state Auditor Crit Luallen, rejected Paul's contention the ad questions his faith.
"This is not about Rand Paul's faith," she said. "It's about his judgment and his character."
Kelley Paul disagrees.
"I could hardly believe my eyes when I first saw it, and neither could our three sons, who are ages 11, 14 and 17," she said.
Kelley Paul, a church deacon, spoke to reporters in a local convention center on her 20th wedding anniversary while her husband, an eye surgeon, treated patients across town.
"I am truly shocked by Conway's ads questioning Rand's character and his faith based on nothing more than anonymous accusations from nearly three decades ago," she said. "Rand and I are both Christians and our faith is very important to us."
Paul said she's hoping that her husband can get back to discussing real issues in the final two weeks of the race.
Paul campaign manager Jesse Benton said his staff decided this morning to arrange for Kelley Paul to speak to reporters to "highlight" the public outcry about the ad, which has been denounced by both Republicans and Democrats. Christian leaders also have criticized the ad, saying many people had done things during their college years that they'd rather not have publicized.
Kelley Paul chided Conway for the "personal, gutter attacks."
"At the end of the day, we all have to look ourselves in the mirror, and I'm proud to say that my husband will be able to do that when this race is over," she said.