A law took effect in Bolivia last year prohibiting all animal performances.
Animal Defenders International is underwriting Wednesday's planned airlift from the steamy lowlands city of Santa Cruz to Denver, where the 13 males and 12 females will move into the nearby Wild Animal Sanctuary.
To acclimatize them, the sanctuary has built a 15,000-square-foot biosphere.
ADI president Jean Cramer says most of the lions were dehydrated and suffered from eye and foot infections when recovered.
It will cost about $8,000 a year to maintain each lion.