More than 1,000 people watched Leno perform Saturday night at the Beau Rivage Resort and Casino in Biloxi to raise money for the Gulf Coast Community Foundation. Tickets were $40 to $150.
At the end of the show, Leno presented an oversized check for $90,336 to foundation president Rich Westfall. Leno chipped in some of his own money, topping off the evening's fundraising total at about $100,000.
Leno is among several celebrities to come to the aid of the Gulf coast, which is losing a large share of its annual tourism money this summer.
Earlier this summer, Jimmy Buffett held a free concrt in Alabama, attended by tens of thousands of people dressed in Hawaiian shirts and parrot hats.
Two weeks ago, President Obama and his family spent a weekiend vacationing on the Florida Panhandle, even swimming in the Gulf to show others how safe it is.